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Biology Laboratory Technician

Published
Time-Based Apprenticeship
Sponsoring Company:
Albany Molecular Research Inc.
O*Net Code
19-4021.00
Rapids Code
Req. Hours
3250
State
NY
Created
Jul 29, 2021
Updated
Jul 29, 2021

Time-Based Skills

5 skill sets | 36 total skills
Safe Work Practices - 100 req. hrs
Develop awareness of work environment and potential biological, chemical, radiological and physical hazards.
Develop awareness of environmental hazards, such as: solids, liquids, gases.
Implement emergency response protocol(s) if necessary.
Select, don, and doff appropriate Personal Protective Equipment (PPE).
Develop awareness and demonstrate compliance with addressing safety and health concerns particular to industry, e.g., nuclear, biological, food, pharmaceutical.
Setup, operate, and store equipment safely.
General Skills - 150 req. hrs
Use Fundamental Laboratory Units and Calculations, including weights, measures, ratios, mixtures, concentration and dilutions.
Accurate, safe weighing of laboratory chemicals.
Prepare solutions of specific concentration and pH.
Accurate, Safe Sample and Reagent Transfer by handheld Pipet.
Calculate and prepare Dilution Series.
Label solutions and wastes per employer procedure.
Sample centrifugation per employer procedure.
Refer to laboratory notebook per employer procedure
Learn and follow ethics and compliance procedures.
Biochemical Testing - 1000 req. hrs
Set-up, use, clean and take-down: Multidrop Combi Liquid Dispenser / ECHO Acoustic Sample Transfer Station / Certus Flex Liquid Dispenser / BioTek Plate Washer / PE EnVision Plate Reader / MD FLIPR Plate Reader.
Implement Biochemical Assay Standard Operating Procedures (SOPs) for sample testing.
Record and analyze biochemical test data.
Cell Culture and Cellular Testing - 1000 req. hrs
Setup, use, and clean: Biosafety Class II cabinets / Cell Incubators / Floor Standing Centrifuges.
Use and clean cell analyzers and microscopes.
Recognize and be familiar with culture and maintenance of: Microbial Organisms / /Insect Cells / Mammalian Cells
Analyze cell culture density and health.
Execute Cell Culture Standard Operating Procedures (SOPs).
Record keeping for cell culture and maintenance.
Execute Cell Toxicity SOPs for sample testing.
Record and analyze Cell Toxicity test data.
Maintain cell culture laboratory housekeeping.
Protein Expression and Purification - 1000 req. hrs
Execute DNA Plasmid, Bacmid and Phage production and analyze SOPs.
Record Keeping for DNA Production.
Setup, use, and clean DNA Production and Analysis equipment.
Execute Cell Culture Infection and Transfection.
Setup, use, and clean Cell Infection, Cell Transfection and
Analysis Equipment.
Execute Protein Production SOPs.
Record Keeping for Protein Production and Analysis.
Setup, use, and clean protein production and analysis equipment.

Technical Instruction

Safety/Health/Environment - 12 req. hrs
General Workplace Safety
Occupational Safety and Health Administration (OSHA) required trainings (if applicable)
Nuclear Regulatory Commission (NRC) training(s) (if applicable)
Proper selection and use of Personal Protective Equipment (PPE)
Biosafety & Bloodborne Pathogens Safety
Industrial Hygiene Plan
High Potency Compound
Procedures Spill Clean-up
Procedures Hazardous Waste
Procedures Radiation Safety
Emergency Response and Evacuation
Hazard Communication Plan
Right-to-Know/Safety Data Sheets (SDS)
First Aid & CPR
Sexual Harassment Prevention Training – MUST comply with section 201-g of the Labor Law
Trade Science - 12 req. hrs
Basic Biochemistry
Basic Microbiology
Basic Cell Biology
Basic Chemistry
Basic Mathematics
Core Protection
Trade Practice - 12 req. hrs
Laboratory Equipment
Biochemical Analysis
Cell Culture and Analysis
Protein Expression, Purification and Analysis
Radiation & Radioactive Decay (if applicable)
Sample Preparation and Testing
Centrifugation
Liquid Handling
Spectrophotometry
Fluorimetry
Liquid Scintillation
Chromatography
Electrophoresis
Bioassay
Workplace Skills - 12 req. hrs
Laboratory Vocabulary
Fundamental Laboratory Units and Calculations, including Weights, Measures, Ratios, Mixtures and Dilutions
Accurate, Safe Weighing of Laboratory Chemicals
Preparation of Solutions of Specific Concentration and pH. Fundamental Hand-Held Pipetting
Calculation and Preparation of Dilution Series
Procedures for Labeling Solutions and Waste
Laboratory Notebook Keeping
Biochemical Testing Skills - 24 req. hrs
Biochemical Vocabulary and Laboratory Orientation
Best Practices for Execution of Biochemical Assay Standard Operating Procedures (SOPs)
Handling and Dilution of Proteins, Nucleic Acids, Substrates and Compounds
Appropriate Actions in the Event of a Deviation from the SOP
Recording and Analysis of Biochemical Test Data
Cell Culture, Maintenance and Test Skills - 24 req. hrs
Cell Culture Vocabulary and Laboratory Orientation
Personal Protective Equipment and Procedures for Cell Culture
Use and Cleaning of Cell Analyzers and Microscopes
Aseptic Technique
How to Read a Cell Culture SOP
Organizing the Workspace and Schedule for SOP Execution
Appropriate Actions in the Event of a Deviation from the SOP
How to Read Microbial and Mammalian Cell Toxicity Assay
SOPs
Protein Expression, Purification and Analysis Skills - 12 req. hrs
Protein Expression, Purification and Analysis Vocabulary and Laboratory Orientation
Execution of DNA Plasmid, Bacmid. P1 Phage, P2 Phage and P3 Phage
Production and Analysis SOPs - 12 req. hrs
How to Read a DNA Production and Analysis SOP
Organizing the Workspace and Schedule for SOP Execution
Production of Plasmid for Expression in E.coli
Production of Plasmid for Expression in Yeast
Production of Plasmid and Bacmid for Expression in Mammalian Cells
Production of Phage for Expression in Insect Cells
Analysis of DNA Samples
Appropriate Actions in the Event of a Deviation from the SOP or an Execution of Cell Infection and Cell Transfection SOPs
How to Read a Cell Infection/Transfection SOP
Organizing the Workspace and Schedule for SOP Execution
Plasmid Transfection of E.coli
Plasmid Transfection of Yeast
Plasmid Transfection of Mammalian Cells
Phage infection of Insect Cells
Assessment of Transfection and Infection Efficiency
Assessment of Target Protein
Appropriate Actions in the Event of a Deviation from the SOP or an unexpected outcome
Record Keeping for Cell Culture Infection and Transfection
Setup, Use and Cleaning of Cell Infection, Cell Transfection and Analysis Equipment
Execution of Protein Production SOPS - 12 req. hrs
How to Read a Protein Production SOP
Organizing the Workspace and Schedule for SOP Execution
Protein Production from E.coli
Protein Production from Yeast
Protein Production from Mammalian Cells
Protein Production from Insect Cells
Assessment of Protein Production Yield and Purity Protein Concentration Assay
Appropriate Actions in the Event of a Deviation from the SOP or an unexpected outcome
Record Keeping for Protein Production and Analysis

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